The haploid pathogenic yeast Candida
glabrata ranks second only to C.
albicans as a cause of bloodstream
fungal infection and candidal vaginitis. One of the most
notable attributes of this species is its decreased susceptibility
to the azole antifungal agent fluconazole.
The C. glabrata MLST scheme was developed
by Andrew Dodgson in the laboratories of David Denning (University
UK) and David Soll (University of Iowa, USA) for the unambiguous
characterization of isolates, and to enable easy inter-laboratory
comparisons to be made. It was first piloted in a study
of 109 geographically diverse isolates.
The allelic profiles
of these strains and others can be found in the database.
Dodgson AR, Pujol C, Denning DW, Soll DR, Fox AJ. Multilocus
sequence typing of Candida glabrata reveals geographically
enriched clades. J Clin Microbiol. 2003 Dec;41(12):5709-17.
The technique has proved useful for studying the population
structure of the species and its worldwide distribution.
It is intended that the technique be used to address
questions about geographical differences seen in
both the incidence
of infection and the antifungal susceptibilities
of isolates. To this end, researchers are encouraged
data to the curator in order that that the database
may be enlarged
and of greater utility to the research community
as a whole.